实验目的:研磨鱼骨提取钙铁锌硒等微量元素。
实验地点:中国农大实验老师:赵新刚(研究方向:动物类饲料的营养及吸收)。
实验前主要用刀片式粉碎机打粉,存在以下问题:
粉碎颗粒大,提取纯度及数量低,影响影响实验效果。
实验材料和器具:
样品:鱼骨;
工具:全自动样品快速研磨仪(JXFSTPRP-24L);
配件:研磨罐、钢珠若干;
研磨条件:冷冻干磨;
实验步骤:
1.鱼骨切小块,放入液氮浸泡5分钟,不锈钢罐液氮浸泡5分钟;
2.将冷冻好的鱼骨样品和钢珠放入研磨罐,并放入适配器中;
3.盖上盖板,固定好旋钮;
4.设定参数,启动仪器至研磨程序结束;
5.研磨完毕后请关闭电源,将安全锁归位。
样品研磨效果如下:
实验解决的问题:各样品之间无污染,微量样品也能研磨,一次处理的样品数量大幅提升,提升工作效率和微量元素的提取率。
注意事项:鱼骨在常温下性质比较疏松有弹性,研磨前需将鱼骨及研磨罐进行液氮冷冻,使鱼骨变得比较脆硬便于研磨。
全自动样品快速研磨仪图片
仪器概述:
上海威九国际的JXFSTPRP-L系列组织研磨仪(专利产品,仿冒必究!ZL2019301918692)采用进口电机,防腐材质,增强型运动轴,采用三维一体8字形振荡方式,研磨更充分,稳定性更好。它是一种特殊的、快速的、高效率的、多试管的一致系统。能将任何来源(包括土壤、植物和动物的组织/器官、细菌、酵母、真菌、孢子、古生物标本等)的原始DNA、RNA和蛋白质进行提取和纯化。?将样品和研磨球放入研磨仪内(配置研磨罐或离心管/适配器),在高频摆动作用下,研磨球在研磨仪内来回高速撞击及摩擦,在非常短的时间内便可完成样品的研磨、粉碎、混合及细胞破壁。
Previous:提取组织器官RNA|威九国际多样品组织匀浆机实验案例
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